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CiteWeb id: 20020000384

CiteWeb score: 2440

DOI: 10.1677/jme.0.0290023

The fluorescence-based real-time reverse transcription PCR (RT-PCR) is widely used for the quantification of steady-state mRNA levels and is a critical tool for basic research, molecular medicine and biotechnology. Assays are easy to perform, capable of high throughput, and can combine high sensitivity with reliable specificity. The technology is evolving rapidly with the introduction of new enzymes, chemistries and instrumentation. However, while real-time RT-PCR addresses many of the difficulties inherent in conventional RT-PCR, it has become increasingly clear that it engenders new problems that require urgent attention. Therefore, in addition to providing a snapshot of the state-of-the-art in real-time RT-PCR, this review has an additional aim: it will describe and discuss critically some of the problems associated with interpreting results that are numerical and lend themselves to statistical analysis, yet whose accuracy is significantly affected by reagent and operator variability.

The publication "Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems" is placed in the Top 10000 of the best publications in CiteWeb. Also in the category Biology it is included to the Top 10000. Additionally, the publicaiton "Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems" is placed in the Top 1000 among other scientific works published in 2002.
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