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CiteWeb id: 19990000212

CiteWeb score: 3360

DOI: 10.1126/science.285.5424.110

An estimated 170 million persons worldwide are infected with hepatitis C virus (HCV), a major cause of chronic liver disease. Despite increasing knowledge of genome structure and individual viral proteins, studies on virus replication and pathogenesis have been hampered by the lack of reliable and efficient cell culture systems. A full-length consensus genome was cloned from viral RNA isolated from an infected human liver and used to construct subgenomic selectable replicons. Upon transfection into a human hepatoma cell line, these RNAs were found to replicate to high levels, permitting metabolic radiolabeling of viral RNA and proteins. This work defines the structure of HCV replicons functional in cell culture and provides the basis for a long-sought cellular system that should allow detailed molecular studies of HCV and the development of antiviral drugs.

The publication "Replication of Subgenomic Hepatitis C Virus RNAs in a Hepatoma Cell Line" is placed in the Top 10000 of the best publications in CiteWeb. Also in the category Biology it is included to the Top 10000. Additionally, the publicaiton "Replication of Subgenomic Hepatitis C Virus RNAs in a Hepatoma Cell Line" is placed in the Top 1000 among other scientific works published in 1999.
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