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CiteWeb id: 19880000007

CiteWeb score: 20124

DOI: 10.1126/science.2448875

A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus, greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Single-copy genomic sequences were amplified by a factor of more than 10 million with very high specificity, and DNA segments up to 2000 base pairs were readily amplified. In addition, the method was used to amplify and detect a target DNA molecule present only once in a sample of 10(5) cells.

The publication "PRIMER DIRECTED ENZYMATIC AMPLIFICATION OF DNA WITH A THERMOSTABLE DNA POLYMERASE" is placed in the Top 1000 of the best publications in CiteWeb. Also in the category Biology it is included to the Top 100. Additionally, the publicaiton "PRIMER DIRECTED ENZYMATIC AMPLIFICATION OF DNA WITH A THERMOSTABLE DNA POLYMERASE" is placed in the Top 100 among other scientific works published in 1988.
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